This technology started to be investigated on vanilla plant since 1990 by collecting and evaluating the potency of several useful microorganisms such as Fusarium oxysporum non-pathogenic, Bacillus, Trichoderma, Penicilium and Pseudomonas fluorescens and plant extract. Since 2001 this technology has started to be launched and developed widely on vanilla organic farming in Indonesia.
Technology of Bio-FOB uses 3 kinds of microorganisms namely non-pathogenic F. Oxysporum and plant extract of clove so that its development orientes to cultivation of organic plants. In cultivation of vanilla plant with technology of Bio-FOB, it does not use anorganic fertilisers and synthetic pesticides. Several investigations show that technology of Bio-FOB can be used on other plants, especially Bio-TRIBA, Organo-TRIBA and Mitol 20 EC likes on cashew plants, black pepper, cocoa, coffee and other vegetables.
First vanili plantation used technology of Bio-FOB, in Tabanan Bali, 2001. Bio-fob vanilla flowers one year early. Productivity higher than non Bio-FOB vanilla, with vanillin rate reach 2,57 – 2,76 % Specification of Technology Components
1) Seedling of Bio-FOB vanilla : Free and tolerant seedling to FR that is produced by using non-pathogenic Fusarium oxysporum (FoNP). This microbe has function to induce system of vanilla resistance to FR and stimulates rooting. This microorganism is isolated from rhizosphere and plant tissue of healthy vanilla. FoNP has a lot of been reported can induce system of plant resistance by the increase of activity several certain enzymes in system of plant metabolism namely β-1,4 glucosidae, β-1,3-glucanase and chitinase. The amount of 15 isolates of FoNP has been succesfully isolated and purifed and one of isolates i.e. FoNP strain F10-AM consistently produce high effectivity. That strain then used to produce 3 kinds of FoNP formula, Bio-FOB EC (liquid), Bio-FOBWP (powder), Organic-FOB (organic materials).
2)Bio-TRIBA : Formula in the form of liquid contains two kinds of microorganism namely B. pantotkenticus and T. lactae. Solution can be used as biodecomposer of organic waste and biofungicide for control of plant pathogen and can be mixed with organic fertiliser in its application. B. Pantotkenticus can stimulate rooting, biodecomposer of raw organic waste and produces antibiotic during decomposition process of organic material and functions as biological agent that will protect rooting system and survives in plant rhizosphere. . T. lactae can decompose raw organic waste to be high quality. T. lactae can inhibit the growth of several pathogenic fungi on plant among others R. lignosus R. solani, F. oxysporum, F. solani, Pythium and S. rolfsii is included the cause of FR disease on vanilla.
3)3) Botanical Fungicide of Mitol 20 EC : Formula that contains active ingredients of eugenol and citrat that were extracted from clove and citronella plants. This compound of eugenol from clove is toxic to several plant pathogens among other R. lignisus, R. solani, F. oxysporum, F. solani,Pythium and S. rolfsi is included pathogen the cause of FR and contaminant fungi on post harvest such as Aspergillus and Penicillium the disease of vanilla FR.
4) Organo-TRIBA. BioOrganic that is processed from selected organic waste from experiment results by using benefit microorganims as biodecomposer. Organo TRIBA contains several useful microorganisms among others Bacillus, Trichoderma, P. fluorescens and Penicillium. Those microorganisms have roles of the increase of health and plant production. Extract of organo-TRIBA compost can be pesticidal on several fungi of soil borne pathogen.
